rotating microarray hybridization oven (Agilent technologies)
Agilent technologies is a verified supplier 
Agilent technologies manufactures this product 
90
Structured Review
Agilent technologies
rotating microarray hybridization oven
Rotating Microarray Hybridization Oven, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+oven/pmc11054952-207-31-34
Average 90 stars, based on 1 article reviews
Rotating Microarray Hybridization Oven, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+oven/pmc11054952-207-31-34
Average 90 stars, based on 1 article reviews
rotating microarray hybridization oven - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes"
Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes
Journal: Non-Coding RNA
doi: 10.3390/ncrna10020025
Figure Legend Snippet: Top ten upregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA microarray dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
Techniques Used: Microarray, Software
Figure Legend Snippet: Top ten downregulated lncRNAs and mRNAs and their respective fold-changes and p -values based on the cDNA microarray dataset. The transcripts were statistically analyzed in the Agilent GeneSpring GX (v14.9.1) software using moderated t -test and Benjamin–Hochberg multiple testing corrections and had satisfied the p -value of <0.05 and fold-change cutoff of ±2.00. Data shown are from four independent experiments.
Techniques Used: Microarray, Software
Figure Legend Snippet: Difference in lncRNA and mRNA fold-changes between the cDNA microarray and in-house RT-qPCR experiments. Data shown are from three independent experiments (in triplicates) with calculated SD values to represent error bars and their statistical analyses were conducted using one-way ANOVA.
Techniques Used: Microarray, Quantitative RT-PCR
Figure Legend Snippet: ( A ) LncRNA-mRNA interaction network was constructed based on the ten selected candidate lncRNAs from the cDNA microarray dataset. The resulting mRNA interactions were predicted using the “rtool” database with −20 kcal as the minimum energy threshold and were filtered to show only mRNAs that were also dysregulated in the dataset. Data were visualized using Cytoscape v3.8.2. (Pink diamond = candidate lncRNA, blue circle = predicted putative mRNA targets). ( B ) A total of nine mRNAs were identified as both responsible in the “regulated exocytosis” hit as well as highly likely to be regulated by the candidate lncRNAs listed in ( A ). Data were visualized using Cytoscape v3.8.2. (pink diamond = candidate regulator lncRNA, blue circle = mRNA predicted involved in the biological process).
Techniques Used: Construct, Microarray
Related Articles
Gene Expression:Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes Article Snippet: Cy3-labeled cRNA was then purified using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) to purify the amplified cRNA samples before dye incorporation and cRNA yield was assessed via NanoVueTM Plus Spectrophotometer (GE Healthcare, Chicago, IL, USA). .. After fragmentation steps following the manufacturer’s protocol, the cRNAs were immediately hybridized into the Agilent SurePrint G3 Human Gene Expression v3 8x60K for 17 h at 65 °C in a rotating Article Title: Homeodomain-only protein suppresses proliferation and contributes to differentiation- and age-related reduced CD8 + T cell expansion Article Snippet: A total of 300 ng Cy3-labeled cRNA and 300 ng of a Cy5-labeled universal standard was fragmented at 60°C for 30 minutes in a reaction volume of 25 μL containing 0.5x Agilent fragmentation buffer and 2x Agilent gene expression blocking agent. .. After fragmentation, 25 μL of 2× Hi-RPM Hybridization Buffer was added to the fragmentation mixture and hybridized to Agilent SurePrint G3 Human GE v3 8x60K Microarray gene expression microarrays for 17 hours at 65°C in a rotating Microarray:Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes Article Snippet: Cy3-labeled cRNA was then purified using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) to purify the amplified cRNA samples before dye incorporation and cRNA yield was assessed via NanoVueTM Plus Spectrophotometer (GE Healthcare, Chicago, IL, USA). .. After fragmentation steps following the manufacturer’s protocol, the cRNAs were immediately hybridized into the Agilent SurePrint G3 Human Gene Expression v3 8x60K for 17 h at 65 °C in a rotating Article Title: Exploring miRNAs’ Based Modeling Approach for Predicting PIRA in Multiple Sclerosis: A Comprehensive Analysis Article Snippet: Samples were hybridized to the Agilent Human miRNA Microarrays chip 8 × 60 K (Agilent PN G4870-60530, grid ID = 070156) containing 2549 human miRNAs. .. The glasses were incubated in the Article Title: Community assembly of organisms regulates soil microbial functional potential through dual mechanisms. Article Snippet: 1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China 2College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, China 3Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, China 4Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China 5College of Land and Environment, Shenyang Agricultural University, Shengyang, China 6Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China 7Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, China 8Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China 9Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, China 10Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, USA Article Title: Multi-tissue metabolic and transcriptomic responses to a short-term heat stress in swine Article Snippet: 600 ng of Cy3-labelled cRNA (speci c activity > 6 pmol Cy3/μg cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 25 μL containing 10x Agilent fragmentation buffer and 25x Agilent blocking agent following the manufacturer’s instructions. .. On completion of the fragmentation reaction, 25 μL of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to SurePrint G3 Mouse GE microarray (8X60K, Design 028005) enclosed in Agilent SureHyb-enabled hybridization chambers for 17 hours at 65°C in a rotating Article Title: Cladribine and ocrelizumab induce differential miRNA profiles in peripheral blood mononucleated cells from relapsing–remitting multiple sclerosis patients Article Snippet: Then, the samples were hybridized to the Agilent Human miRNA Microarrays chip 8x60K (Agilent PN G4870-60530, grid ID = 070156) containing 2,549 human miRNAs. .. The glasses were incubated in the Article Title: Targeting microRNA-145-mediated progressive phenotypes of early bladder cancer in a molecularly defined in vivo model Article Snippet: RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent). .. RNA samples were labeled with the Agilent Low Input Amp Labeling Kit (Agilent, cat. no. 5190-2305) and hybridized on slides of SurePrint G3 Human GE Microarray 8x60K version 3.0 (Agilent) in the Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay Article Snippet: Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent). .. For hybridization, DNA microarrays were placed into a Article Title: Homeodomain-only protein suppresses proliferation and contributes to differentiation- and age-related reduced CD8 + T cell expansion Article Snippet: A total of 300 ng Cy3-labeled cRNA and 300 ng of a Cy5-labeled universal standard was fragmented at 60°C for 30 minutes in a reaction volume of 25 μL containing 0.5x Agilent fragmentation buffer and 2x Agilent gene expression blocking agent. .. After fragmentation, 25 μL of 2× Hi-RPM Hybridization Buffer was added to the fragmentation mixture and hybridized to Agilent SurePrint G3 Human GE v3 8x60K Microarray gene expression microarrays for 17 hours at 65°C in a rotating Hybridization:Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes Article Snippet: Cy3-labeled cRNA was then purified using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) to purify the amplified cRNA samples before dye incorporation and cRNA yield was assessed via NanoVueTM Plus Spectrophotometer (GE Healthcare, Chicago, IL, USA). .. After fragmentation steps following the manufacturer’s protocol, the cRNAs were immediately hybridized into the Agilent SurePrint G3 Human Gene Expression v3 8x60K for 17 h at 65 °C in a rotating Article Title: Exploring miRNAs’ Based Modeling Approach for Predicting PIRA in Multiple Sclerosis: A Comprehensive Analysis Article Snippet: Samples were hybridized to the Agilent Human miRNA Microarrays chip 8 × 60 K (Agilent PN G4870-60530, grid ID = 070156) containing 2549 human miRNAs. .. The glasses were incubated in the Article Title: Community assembly of organisms regulates soil microbial functional potential through dual mechanisms. Article Snippet: 1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China 2College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, China 3Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, China 4Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China 5College of Land and Environment, Shenyang Agricultural University, Shengyang, China 6Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China 7Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, China 8Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China 9Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, China 10Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, USA Article Title: Multi-tissue metabolic and transcriptomic responses to a short-term heat stress in swine Article Snippet: 600 ng of Cy3-labelled cRNA (speci c activity > 6 pmol Cy3/μg cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 25 μL containing 10x Agilent fragmentation buffer and 25x Agilent blocking agent following the manufacturer’s instructions. .. On completion of the fragmentation reaction, 25 μL of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to SurePrint G3 Mouse GE microarray (8X60K, Design 028005) enclosed in Agilent SureHyb-enabled hybridization chambers for 17 hours at 65°C in a rotating Article Title: Cladribine and ocrelizumab induce differential miRNA profiles in peripheral blood mononucleated cells from relapsing–remitting multiple sclerosis patients Article Snippet: Then, the samples were hybridized to the Agilent Human miRNA Microarrays chip 8x60K (Agilent PN G4870-60530, grid ID = 070156) containing 2,549 human miRNAs. .. The glasses were incubated in the Article Title: Targeting microRNA-145-mediated progressive phenotypes of early bladder cancer in a molecularly defined in vivo model Article Snippet: RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent). .. RNA samples were labeled with the Agilent Low Input Amp Labeling Kit (Agilent, cat. no. 5190-2305) and hybridized on slides of SurePrint G3 Human GE Microarray 8x60K version 3.0 (Agilent) in the Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay Article Snippet: Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent). .. For hybridization, DNA microarrays were placed into a Article Title: Homeodomain-only protein suppresses proliferation and contributes to differentiation- and age-related reduced CD8 + T cell expansion Article Snippet: A total of 300 ng Cy3-labeled cRNA and 300 ng of a Cy5-labeled universal standard was fragmented at 60°C for 30 minutes in a reaction volume of 25 μL containing 0.5x Agilent fragmentation buffer and 2x Agilent gene expression blocking agent. .. After fragmentation, 25 μL of 2× Hi-RPM Hybridization Buffer was added to the fragmentation mixture and hybridized to Agilent SurePrint G3 Human GE v3 8x60K Microarray gene expression microarrays for 17 hours at 65°C in a rotating Incubation:Article Title: Exploring miRNAs’ Based Modeling Approach for Predicting PIRA in Multiple Sclerosis: A Comprehensive Analysis Article Snippet: Samples were hybridized to the Agilent Human miRNA Microarrays chip 8 × 60 K (Agilent PN G4870-60530, grid ID = 070156) containing 2549 human miRNAs. .. The glasses were incubated in the Article Title: Cladribine and ocrelizumab induce differential miRNA profiles in peripheral blood mononucleated cells from relapsing–remitting multiple sclerosis patients Article Snippet: Then, the samples were hybridized to the Agilent Human miRNA Microarrays chip 8x60K (Agilent PN G4870-60530, grid ID = 070156) containing 2,549 human miRNAs. .. The glasses were incubated in the Labeling:Article Title: Community assembly of organisms regulates soil microbial functional potential through dual mechanisms. Article Snippet: 1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China 2College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, China 3Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, China 4Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China 5College of Land and Environment, Shenyang Agricultural University, Shengyang, China 6Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China 7Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, China 8Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China 9Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, China 10Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, USA Article Title: Targeting microRNA-145-mediated progressive phenotypes of early bladder cancer in a molecularly defined in vivo model Article Snippet: RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent). .. RNA samples were labeled with the Agilent Low Input Amp Labeling Kit (Agilent, cat. no. 5190-2305) and hybridized on slides of SurePrint G3 Human GE Microarray 8x60K version 3.0 (Agilent) in the |
